SubtiBank SubtiBank
ykuL [2019-04-30 16:05:15]
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ykuL [2019-04-30 16:05:15]

unknown
Locus
BSU14130
Isoelectric point
5.16
Molecular weight
16.49 kDa
Protein length
147 aa Sequence Blast
Gene length
444 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,485,453 1,485,896

The protein

Domains

  • CBS domain (18-78)
  • Structure

    Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • Spo0A: activation, by Spo0A~P PubMed, in Spo0A regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
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    Biological materials

    Mutant

  • MGNA-A773 (ykuL::erm), available at the NBRP B. subtilis, Japan
  • GP2769 (ΔdarB::ermC), available in Jörg Stülke's lab
  • BKE14130 (ykuL::erm trpC2) available in the Bacillus Genetic Stock Center and in Jörg Stülke's lab PubMed
  • GP214 (deletion of ykuJ-ykuK-abbA-darB, replaced by aphA3), available in Jörg Stülke's lab
  • BKE14130 (darB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
  • BKK14130 (darB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
  • GP2800 (ΔdarB-ccpC]::phleo), available in Jörg Stülke's lab
  • Expression vectors

  • pGP2929 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
  • pGP635: expression of Strep-ykuL by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
  • pGP767: expression of ykuL-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
  • pGP1550: expression of ykuL by pBQ200 in B. subtilis, available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP2810 ykuL-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
  • References

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